From the South (Twin Cities area)
From I-35 North take exit 256B (5th Avenue West). At the lights turn right on Harbor Drive. The Aquarium is 2 blocks on your right.
From the North East (North Shore area)
Take Highway 61 to London Road (Highway 61 turns into London Road as you enter Duluth). At 26th Avenue East take I-35 South to exit 256B. Keep right to 5th Avenue West. At 5th Avenue West/Harbor Drive lights turn left. The Aquarium is 3 blocks on your right.
From the East (Wisconsin)
Take Highway 53 North to I-35 North. Take exit 256B (5th Avenue West). At the lights turn right on Harbor Drive. The Aquarium is 2 blocks on your right.
From the North West (Iron Range cities)
Follow signs for Highway 53 South to I-35 North. Take Northbound I-35 to exit 256B (5th Avenue West). At the light, turn right on Harbor Drive. The Aquarium is 2 blocks on your right.
NOTE: Construction continues on Interstate 35. This may impact those traveling to the Aquarium from south and east of Duluth. Visit the Minnesota Department of Transportation website for a complete list of projects and potential delays before you travel.